RNA

Part:BBa_K3602007:Design

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


rs6983267-G Target Cas13a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The T7 promoter was chosen from neb.com for high yield RNA synthesis. The gene sequence itself was looked up on dbSNP.

Source

The rs16901979 is taken from SNPedia https://www.snpedia.com/index.php/Rs6983267) and the T7 promoter is from neb.com (https://international.neb.com/protocols/2015/11/24/sgrna-synthesis-using-the-hiscribe-quick-t7-high-yield-rna-synthesis-kit-neb-e2050).

References